prominent and similar to levels found in previously-published mDA cultures. EN1 levels are lower, but well above the limit of detection, and were also observed in cultures of excitatory neuron precursors (NSC0 and NSC5). Expression plots for several other groups of midbrain-specific and contrasting cortical-specific genes are depicted in Supplemental Fig. 2A–G. All results support the relationship shown in Fig. 3A, that the N398 and D398 cultures are most similar to the previously-published mDA cultures. We conclude that gene expression patterns are consistent with the presence of midbrain-like DA neurons in our cultures.
