For conventional immunofluorescence (e.g. Fig. 1A), cells were seeded onto coverslips 24 h prior to fixation using 4% paraformaldehyde in PBS and permeabilisation with 0.1% Triton X-100 in PBS. After labelling with primary and secondary antibodies (diluted in PBS with 3% BSA), the coverslips were mounted onto glass slides with ProLong mounting medium (Invitrogen). Cells were imaged using a Zeiss AxioPlan microscope with a ×63 PlanAPO objective lens. Images were captured through a Hamamatsu CCD camera controlled via the manufacturer's software.
