Neurotransmitter-specific gene expression allows for a global assessment of the impact of ethanol on the brain. The impact of the ethanol binge drinking procedure was assessed in both adolescent and young adult groups one day after treatment and 50 days later. There was a significant main effect of AE binge treatment (P28-37, Figure 1A) on neurotransmitter-specific gene expression 24 hours after treatment on P38 (Figure 2C; average reduction of 57%, p<0.0001, F=161.2). However, there was not a significant main effect of binge ethanol treatment during adulthood (P88-97) on neurotransmitter-specific gene expression 24 hours after treatment (Figure 2D; p = 0.39, F=0.74). AE binge enhanced the developmental downregulation of neurotransmitter-specific genes. A significant main effect of AE treatment was observed (p<0.0001, F=650.9), with a larger percent reduction than controls (73% reduction vs. 57% in controls). Of the 73 neurotransmitter-specific genes measured in the array, 58 were persistently reduced by binge ethanol treatment at P88 (Table 1, *p<0.05, **p<0.01 t-test, P38 control vs P88 ethanol), and 31 after Bonferroni correction for multiple comparisons (Table 1, §p<0.05 Bonferroni post-test). Regarding the 15 cholinergic
