Monocytes were purified from donor blood cones from two healthy volunteers, and 5 × 106 cells were used per stimulation assay (n = 6). Cells were either untreated or treated as per LPS2 and IFNγ stimulations for the eQTL analysis. At the end of the experiment, cells were cross-linked and pellets were frozen. ChIP was performed with cross-linked cells lysed on ice in a lysis buffer [140 mM NaCl, 50 mM Hepes (pH 7.5), 1 mM EDTA (pH 8), 0.5 mM EGTA (pH 8), 0.5% (v/v) NP-40, 0.25% (v/v) Triton X, and 10% glycerol]. Lysis buffer was supplemented with 1× Complete Protease Inhibitor Cocktail (Roche), 0.5 mM phenylmethylsulfonyl fluoride (Sigma), pepstatin (10 μg/μl) (Sigma), and leupeptin hemisulfate (10 μg/μl) (Sigma) before usage. Nuclear pellets were collected after brief centrifugation and washed using a buffer [10 mM tris (pH 8), 1 mM EDTA (pH 8), 2% (v/v) Triton X, 0.2% (v/v) Na deoxycholate, 10% glycerol] similarly supplemented as described above. Sonication to fragment cross-linked chromatin was carried out using the Bioruptor (Diagenode) at high power over 12 cycles of 30 s
