Where ∑Q is the sum of + IR cells counted from each dissector frame, ∑dissector is the sum of the number of dissector frames counted, A(fr) is the known area associated with each dissector frame, and h is the known distance between two dissector planes (10 μm was used). For co-labeling study, double-stained sections were digitally photographed with Leica (Wetzlar, Germany) SP2-AOBS confocal microscope and analyzed with Leica SP2 LCS software.
