10−3) but not in non-somatic processes (Fig. 5J.g, p = 0.23). Ethanol increased KCNJ6 mRNA levels in both groups (Fig. 5J.a–e), eliminating the difference between genotypes. Interestingly, we also observed that the proportions of AF neurons expressing KCNJ6 was increased (8.3%, Fig. 5J.b) by ethanol and was paralleled by a reduction in number of low-expressing cells and increased appearance of high-expressing neurons (Fig. 5J.c). Results indicate that variant KCNJ6 haplotype leads to reduced KCNJ6 expression, which is consistent with RNA sequencing and immunocytochemical results, and that ethanol increases KCNJ6 expression, eliminating differences between haplotype groups.
