and activating the SMD pathway.46, 47, 48 Using the bioinformatics software RepeatMasker and IntaRNA, we predicted the putative SBS between the Alu element on LINC00665 and the Alu element on either the MTF1 or YY2 mRNA 3′ UTR, which was confirmed by luciferase gene reporter assays. The RNA-IP assay confirmed the associations among LINC00665, STAU1, and MTF1(YY2), and both LINC00665 overexpression and STAU1 inhibition influenced MTF1 and YY2 mRNA degradation based on half-life assays. Regulation networks of the SMD pathway involving lncRNAs have attracted increasing research attention. SNHG5 was reported to be upregulated in colorectal cancer cells and to participate in STAU1-mediated mRNA destabilization.49 In addition, lncRNAs participating in the SMD pathway were suggested to play a role in the pathogenesis of ischemic stroke.50
