for cultures that model neurological or psychiatric disorders and require resolution of individual neurons or defined networks, both of which are challenging because cells tend to form dense clusters during the differentiation process. Several approaches are in development to address these limitations, including the use of physical barriers that guide or compartmentalize neurons [6-8], chemically patterned substrates [9-10], and microfluidic devices that offer control over the positions and local environment surrounding the cells [11-12]. These methods, however, are not always straightforward to implement and have largely been limited to the patterning of primary neurons for only a few weeks [13-15]. For example, Zhang and coworkers reported three week cultures of cortical cells on polymeric substrates modified with a aminopropyl siloxane [16] and West and coworkers reported month-long cultures of primary neurons on glass slides patterned with poly(ethylene glycol) [17]. Microcontact printing of self-assembled monolayers of alkanethiolates on gold has also been used to pattern adherent cells [18]. The patterned monolayers have the benefits that they are straightforward to prepare and they maintain the patterned cells from one to four weeks [19-21]. In this paper we demonstrate that microcontact printing can be used to prepare patterned monolayers that maintain the positions
