To determine whether DLX2 directly regulates enhancers 649 or 675, we performed chromatin immunoprecipitation (ChIP)-qPCR of E13.5 basal ganglia using a DLX2 antibody. We found enrichment over several homeodomain-containing regions of enhancers 649 and 675, with a particular domain of #675 (region #3) showing the strongest enrichment as compared to control regions of the of the genome (Figure 5K). Also, the relative enrichment of the enhancer fragments was eliminated when a DLX2 polypeptide was included in the immunoreaction as a negative control (Figure 5K).
