The effects of the ethanol treatment on changes in protein levels in the ACB-shell of W rats (Table 5) indicated that few similar alterations were occurring in the W rats as were evident in NP rats (Tables 3 and 5). There were some changes in the levels of proteins that were observed for the W rats that suggested possible alterations in neuronal function; some of these changes were also observed for the P rat (Tables 2 and 5). For example, there were increased protein levels for NipSnap 2, involved in vesicular transport (Seroussi et al., 1998), and Synpo and Ngef, which are involved in postsynaptic function (Okubo-Suzuki et al., 2008; Sakagami et al., 2008). The overall results suggest that W rats may be undergoing ethanol-induced neuronal alterations in the ACB-shell, and that some of these alterations may be similar to changes observed for P, but different than changes observed for NP rats.
