Primary microglia and cell lines have been maintained for short periods on canonical polymer linkers such as poly-D-Lysine (PDL) + Laminin, but this is also a substrate for neural progenitors, neurons and glia. To exclude the growth of neuro-ectodermal derivatives we used Primaria plastic to positively select pMGLs. It is notable that coatings such as PDL or matrigel can represent another layer of variability between cultures, and their exclusion contributes to the reproducibility of this protocol.
