Handling-induced convulsions were determined using a modified scoring paradigm from Buck et al. (1997). Briefly, each mouse was gently picked up by the tail and spun, if required, to determine a baseline convulsion score. Mice were then injected, i.p., with 4.0 g/kg ethanol (20%) and HIC scores re-determined at 4, 6 and 7 h post-injection. HIC scores were the combined difference scores (i.e. baseline score subtracted from the later scores) for each value following the injection.
