Chunk #63 — SPECIFIC MECHANISMS OF TUMOR SUPPRESSION AND THERAPEUTIC TARGETING — Synergy between TopoII and BAF complexes

Source

Mammalian SWI/SNF chromatin remodeling complexes and cancer: Mechanistic insights gained from human genomics.

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using etoposide, it was possible to capture the enzyme covalently attached to DNA before the religation step could be completed. Using this approach, the Brg (Smarca4) ATPase was found to be essential for the binding of TopoII to 11,000 of 16,000 sites over the genome (97). TopoII was found to bind to the BAF250a subunit, which is the one most frequently mutated in human cancer.