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Chunk #4 — Methods and Materials — The Selection Phenotype

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A line of mice selected for high blood ethanol concentrations shows drinking in the dark to intoxication.
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We wanted to select mice drinking to intoxication without behaviorally testing them for intoxication. Because intake was imperfectly predictive of BEC and because the many lines selected for high intake rather than for intoxication do not readily drink to intoxicating BECs, we elected to breed selectively on BEC attained rather than intake in g/kg.