Tissue sections on positively charged slides were processed for haematoxylin-eosin staining by dipping the slides in a series of baths as follow: distilled water (30 s), haematoxylin (5 min); distilled water (1 min); 0.5% eosin Y (2 min); 95% Ethanol (30 s × 2); 100% ethanol (30 s × 2); and xylene (30 s × 2). Depex mounting medium and glass cover slips were used to mount the slides. Slides were analysed by using an upright microscope (Olympus).
