at near background levels, only genes that had 2 or more CPM in at least 5 samples were retained for analysis (cf. (McClintick & Edenberg, 2006). Paired analysis was done based on experimental replicate: ethanol vs. controls, ethanol vs. ethanol withdrawal, and ethanol withdrawal vs. controls. False discovery rates (FDR) were calculated using the method of Benjamini & Hochberg (Benjamini & Hochberg, 1995) within edgeR.
