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Chunk #6 — Methods and materials — Subjects

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The mu-opioid receptor gene and smoking initiation and nicotine dependence.
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yes

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The buccal epithelial cell samples were collected using standard cytology brushes (Fisher Scientific, Fair Lawn, New Jersey). DNA was isolated from the brushes as reported previously [24]. All individuals provided informed consent for participation in this study. Sixteen unlinked microsatellite markers were genotyped to assess the potential population stratification and no evidence of stratification was found [24].