Protein expression was examined by Western blotting. Cell lysates (50 μg) were prepared in RIPA buffer…The following antibodies were used: mouse monoclonal antibodies to E-cadherin (Santa Cruz, CA, USA), Vimentin (Santa Cruz), β-actin (Boster Biotechnology, Wuhan, China) and rabbit polyclonal antibodies against GLI1, SNAI1, N-cadherin and CtBP2 (Abcam, Cambridge, UK).
