SCNN1A, were re-methylated and decreased in expression levels whereas fibroblast-specific promoters such as TMEM173, EMILIN1, LMNA, and RIN2 were demethylated and regained expression in fibroblast-like cells (Fig. 1D). In a final step, the fibroblast-like cultures were reprogrammed into hiPSCs by infecting the cells with SeV vectors expressing OKSM, as previously reported19 (Fig. 1A). Emerging colonies were isolated after ~3 weeks, expanded and confirmed to be positive for AP activity and endogenous OCT4 expression, indicating successful reprogramming (Fig. 1C). Moreover, we ensured loss of SeV expression in all lines, demonstrating reprogramming factor independent self-renewal (Supplementary Fig. 1C,D).
