Chunk #71 — PROCEDURE — Functional testing: detection of indel mutations by the SURVEYOR nuclease assay ● TIMING 5–6 h

Source

Genome engineering using the CRISPR-Cas9 system.

Embedded

yes

Text

? TROUBLESHOOTING 78Purify the PCRs with the QIAQuick PCR purification kit, and normalize the eluted product to 20 ng μl–1.