Chunk #15 — Materials and methods — Cell culture and treatments — Nrf2 gene over expression in HepG2 cells

Source

High Fat Diet-Induced Hepatic 18-Carbon Fatty Acids Accumulation Up-Regulates CYP2A5/CYP2A6 via NF-E2-Related Factor 2.

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pcDNA3-EGFP-C4-Nrf2 and pcDNA3-EGFP-C4 were the Nrf2 over expression plasmid and the NC plasmid, respectively. They were both bought from Addgene. The procedures of transfection and cells treatments are same as Section Nrf2 Gene Silence in HepG2 Cells.