in the more posterior regions of the basal forebrain from bregma + 0.02 to − 0.22 (Figure 9A, *p<0.05, t-test). Examples of two of the measured regions of interest in the posterior basal forebrain are shown (Figure 9B, C). Reduced histological basal forebrain area supports the observed MRI volume reduction in the posterior basal forebrain. Thus, adolescent binge ethanol treatment results in reduced volume of adult olfactory bulb and basal forebrain.