Chunk #47 — MATERIALS AND METHODS — Metabolic labeling of proteoglycans and GAG analysis

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Musculocontractural Ehlers-Danlos syndrome and neurocristopathies: dermatan sulfate is required for Xenopus neural crest cells to migrate and adhere to fibronectin.

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The control and lyase-treated, labeled PGs were analyzed by SDS-PAGE as previously described (Pacheco et al., 2009b); alternatively, they were applied to a size-permeation Superose 6 column (GE Healthcare) and run in the urea-containing buffer as previously described, and the eluted fractions were counted by β-scintillation.