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Chunk #15 — 2. Methods — 2.2. Genotyping

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Associations of glutamate decarboxylase genes with initial sensitivity and age-at-onset of alcohol dependence in the Irish Affected Sib Pair Study of Alcohol Dependence.
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All genotyping was conducted in Dr. David Goldman's Laboratory. Genotyping was performed using the Illumina GoldenGate genotyping protocols on 96-well format Sentrix® arrays and 500 ng of sample DNA were used per assay. All pre-PCR processing was performed using a TECAN liquid handling robot running Illumina protocols. Arrays were imaged using an Illumina Beadstation GX500 and the data analyzed using GenCall v6.2.0.4 and GTS Reports software v5.1.2.0 (Illumina).