Chunk #17 — Materials and Methods — Electrophysiology

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Examining the effects of alcohol on GABA receptor mRNA expression and function in neural cultures generated from control and alcohol dependent donor induced pluripotent stem cells.

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All electrophysiological recordings were performed using a HEKA EPC9 amplifier and PatchMaster software (version 2×67). Analysis and quantification of peak GABA-mediated currents was performed using Axon Clampfit software (version 10.3.1.4).