Biosystems, Foster City, CA; catalog number 4333765F, FAM-labeled). Reactions were carried out in triplicate in a 384-well LightCycler 480 (Roche Applied Science) in reaction volumes of 8 uL, with 5 ng of reverse-transcribed RNA, 1x final concentration of Roche LightCycler 480 Probes master mix, 450 nM of each primer, and 125 nM of fluorescent probe. Assay efficiencies were determined using two-fold serial dilutions of pooled cDNA. Relative expression levels for PBRM1 were calculated using the efficiency-corrected comparative threshold method 28. We used the sample with the median PBRM1 expression level as calibrator and log2-transformed expression levels. One hundred samples were successfully assayed. (As recommended by SMRI, one case sample was omitted due to a known degenerative neurological disorder). Of the remaining 99 samples (31 with bipolar disorder, 34 with schizophrenia, and 33 healthy controls), 97 were from donors of European ancestry. Ninety-six of these samples were successfully genotyped at rs2251219 with standard exonuclease methods (TaqMan, Applied Biosystems, Boston, MA). Data were analyzed by ANOVA (Xlstat 6.0), with diagnosis as the independent, and relative expression level as the dependent, variable. Specific comparisons were performed with the Tukey HSD test. The entire data set has been uploaded to the SMRI data bank.
