CCK and Reelin (RELN); however, only CB, CR and SST were consistently expressed in all biological replicates of AMD- iN cells (Fig. 2g(ii)). The expression pattern of these interneuron markers was very similar in AD-iN cells, except that RELN and VIP were also consistently detected. Finally, we compared the expression pattern of transcription factors important for GABAergic neuron specification and found that LHX6, ZEB2 and SATB1 (but not NKX2-1) expression was consistently detected in AMD- and AD-iN cells (Fig. 2g(iii)). Since LHX6 and SATB1 were detectable in primary cultures of mouse glia, we verified their expression in FACS- purified AD-iN cells. These results show that AMD- and AD-iN cells have a similar morphology and gene expression pattern.
