SCZD hiPSCs compared to control hiPSCs. However, the expression levels of NEUROD1 and PROX1 in the SCZD lines were significantly lower compared to the controls (Figures 6D and 6E). Furthermore, the expression of TBR1, a transcription factor found in postmitotic DG neurons (Hodge et al., 2012), was also significantly reduced in the SCZD neurons (Figure 6F), indicating that there might be defects in the generation of DG granule neurons from the NPC population. Interestingly, the expression of FOXG1 was also lower in the SCZD lines than in the controls (Figure 6C). Because FOXG1 has been previously shown to play important roles in both NPC proliferation and survival of newborn DG granule neurons (Shen et al., 2006), this finding was suggestive of potential contributing factors leading to the deficits in hippocampal neurogenesis by the SCZD lines.
