Chunk #50 — MATERIALS AND METHODS — Single cell gene expression (BD Resolve system)

Source

Assembly of functionally integrated human forebrain spheroids.

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yes

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Sequencing libraries were quantified using a High Sensitivity DNA Chip (Agilent) on a Bioanalyzer 2100 and the Qubit High Sensitivity DNA Assay (Thermo Fisher Scientific). 1.5 pM of the library for each sample was loaded onto a NextSeq 500 system and sequenced using High Output sequencing kits (75 × 2bp) (Illumina).