The Qiagen RNeasy and Lipid Tissue kit (Qiagen, Valencia, CA, USA) was used to extract total RNA from human PFC brain tissue, and RNA concentration was measured with a NanoDrop 8000 spectrophotometer (ThermoFisher Scientific). An Agilent Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) was used to test the integrity of RNA samples. Samples with an RNA integrity number (RIN) < 5.5 were removed from futher analyses. Sixty samples were processed at the Waggoner Center for Alcohol and Addiction Research (WCAAR), The University of Texas at Austin while 83 samples were processed at the Ronald M. Loeb Center for Alzheimer disease, Icahn School of Medicine at Mount Sinai. Details about the library preparation and sequencing is provided in the supplementary document.
