micromole SNAP after subtracting blank values (media alone) from samples and standard values. The effect of EtOH/Ach (17.5 mM EtOH or 10 μM Ach) on iNOS induction in neurons was determined by NO production using the iNOS-specific inhibitor, L-NAME (50 μM, NG-nitro-L-arginine methyl ester). The novelty of NO or ROS detection assay described in the present studies is the inclusion of a positive standard curve for each assay.
