and Broad subsets, and within the SSC control trios (Figure S4). We focused on the first four principal components (PCs), which explain 61.6% of the variance in the quality metrics. We considered samples greater than three standard deviations (SD) from the mean in any of the first four principal components outliers, and consequently, we removed from the analysis the entire family containing that sample (n = 23 of 1219 families or 1.89% of all families; Table S1; Figure S4).
