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Chunk #26 — Methods — mRNA expression data

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Tobacco smoking is associated with methylation of genes related to coronary artery disease.
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yes

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Illumina gene expression data was quantile normalized to the median distribution and subsequently log2 transformed. The probe and sample means were centred to zero. Genes were declared significantly expressed when the detection p-values calculated by Genome Studio were less than 0.05 in more than 10 % of all discovery samples, which added to a total number of 21,238 probes. Quality control was done using the eQTL mapping pipeline.