± 3.9%; iPS2, 92.7 ± 3.3%) and very little overlapping PDGFRα expression (ESC, 6.1 ± 2.7%; iPS1, 7.2 ± 4.5%; iPS2, 5.4 ± 2.6%; Fig. 1E, 1F). By week 3 of differentiation, the number of O4+ cells remained at levels similar to those seen at week 1 across all lines (p ≥ 0.3, unpaired t tests). PDGFRα+‐OPCs and O4+/MBP+‐oligodendrocytes displayed distinct morphology, with OPCs being morphologically less complex whereas oligodendrocytes possessed a radial and multibranched morphology (Fig. 1F). Sholl analysis also revealed an increase in number and complexity of O4+/MBP+ oligodendrocyte processes from week 1 to 3 (Fig. 1G).
