Chunk #8 — MATERIALS AND METHODS — Liver Histology and Immunohistochemistry — Cytochrome P450 2E1 and 2A5 activity

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Absence of cytochrome P450 2A5 enhances alcohol-induced liver injury in mice.

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CYP2E1 activity was measured by the rate of oxidation of 1 mM p-nitrophenol (18), while CYP2A5 activity was measured by assessing COH activity with 100 μM coumarin as substrate (9, 12). The reaction systems were incubated with 100 μg of microsomal protein for 15 min at 37 °C.