We next took a more direct approach to examine relationships between regions of the brain based on dissimilarity of gene expression, by tabulating genes exhibiting highly differential expression between all pairs of regions. Significant pairwise differential relationships (BH-corrected P < 0.01) were independently recorded in each brain and a threshold set for at least a 2.8-fold ratio between structures (Supplementary Table 5). Figure 4a illustrates the resulting neuroanatomical molecular ‘blueprint’ common to both brains, by plotting the number of genes differentially expressed between each pair of structures based on the 11,414 genes passing these criteria in both brains (individual brain maps in Supplementary Fig. 3).
