the same 11 SNPs had p-values among the top 200 associations in unadjusted analyses as well as with adjustment via principal components and stratified analyses; and (b) for the 57 SNPs in the PCLO region, the p-values across these three types of analyses were consistent (the Spearman correlations between p-values from trend tests, logistic regression, and stratified analyses were all >0.962). Sixth, the minor allele frequencies in the control group in the PCLO region were usually quite similar to available EUR control groups suggesting that the PCLO findings were not due to an artifact of the control selection process (see below). Finally, bioinformatic investigation did not suggest that this is a problematic region to genotype as the PCLO region is not known to be under positive selection in humans (74), to contain segmental duplications (67), or common copy number variants (search of the Database of Genomic Variants yielded two rare CNVs with control frequencies of 0.12% and 0.89%) (75, 76 , 77).
