Chunk #31 — Experimental Procedures — Gene Expression Profiling

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Genetic Variability Overrides the Impact of Parental Cell Type and Determines iPSC Differentiation Potential.

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Microarray data were confirmed using qPCR. cDNAs for qPCR reactions were produced from the extracted RNA samples (2 μg) by Maxima First Strand cDNA synthesis kit (Thermo Scientific). qPCR was performed with EvaGreen qPCR mix (Solis Biodyne) in Corbett RotorGene. Primers used NANOG F/R (CTC AGC CTC CAG CAG ATG C/TA GAT TTC ATT CTC TGG TTC TGG); SOX17 F/R (CCG AGT TGA GCA AGA TGC TG/T GCA TGT GCT GCA CGC GCA); SNAI2 F/R (GGT TGC TTC AAG GAC ACA TTA G/TT GAC CTG TCT GCA AAT GCT C); MEG3 F/R (AAG GAC CAC CTC CTC TCC AT/A GGA AAC CGT GCT CCT AGT G); and WNT3A (GCC CCA CTC GGA TAC TTC T/GG CAT GAT CTC CAC GTA GT).