these modules may represent different RG populations. Fluorescent ISH (FISH) confirmed that representative genes in both G7 (NR2E1) and G8 (SPATA13) are enriched in VZ versus SZ (Fig. 3f; Extended Data Fig. 7). Surprisingly, rather than labeling distinct cell populations in VZ, these genes labeled mutually exclusive subcellular locations within the same VZ cells (note non-overlapping cytoplasmic localization of SPATA13 and NR2E1), suggesting that these modules represent differentially regulated biological processes within RG cells.
