layer retained relatively immature characteristics, comparable to the measurements seen in the younger 14–16 DIV neurons plated onto a mouse feeder layer. The RMP, action potential rise time, action potential half width, and input resistance were significantly different in the cortical feeder condition when compared to the 10-18 neurons on mouse feeders recorded at the same DIV range. Sample traces depicting qualitative firing properties under the various conditions are shown in Figure S6.
