The ion homeostasis and sophisticated intrinsic and synaptic physiology properties are shaped by several families of voltage-gated ion channels (VGICs), each contains diverse family members with characteristic biophysical properties (Yu and Catterall, 2004). We demonstrate extensive differential transcription profiles within and across multiple VGICs families among PCPs (Figure S4). Within the Nav and Cav family, major pore-forming subunits are broadly expressed among PCPs, often with different expression levels (Figure S4C–E). Cav auxiliary subunits (β1–2, α2, δ1–4) show more distinct, often binary pattern (Figure S4D), suggesting cell specific regulation of the trafficking, gating, and kinetics of pore forming subunits. Within the Kv family, different subsets are differentially enriched among PCPs (Figure S4C); there is often a tight correlation between expression of principle subunits (e.g. Kcna1/Kv1.1 and Kcna2/Kv1.2) and their matching auxiliary subunits (Kvβ1–3, Kcnab1, b2 & b3) in specific PCPs (e.g. PVBCs; Figure S4), implying cell specific assembly of functional channel complex. These results suggest that differential and correlated expression across multiple families of VGIC subunits may customize electrical signaling among PCPs (Figure S4C–G).
