To link our findings further to existing animal literature, we performed computational haplotype-based genetic mapping analyses48–50 of data from 23 inbred mouse strains for a robust measure of opioid physical dependence,48 counts of jumps made by morphine dependent mice after naloxone administration. Correlations were calculated for the distribution of the mean number of jumps per strain with known haplotype blocks across strains for CNIH3 and genes encoding AMPAR subunits and proteins involved in alterations of AMPAR subunit composition in response to opioids. Significant correlation was observed for CNIH3 haplotype, but not for the more widely expressed cornichon family AMPA receptor auxiliary protein 2 (CNIH2) (Table 3). Significant correlations were also noted for haplotypes in glutamate receptor, ionotropic, AMPA 1 (GRIA1), glutamate receptor, ionotropic, AMPA 2 (GRIA2), calcium channel, voltage-dependent, gamma subunit 8 (CACNG8), and glutamate receptor-interacting protein 1 (GRIP1), but not discs, large homolog 4 (DLG4)].
