pair with some sensitive synaptic staining protocols, many commercial MEA plates are not amenable to imaging, and post hoc FISH would dramatically reduce the throughput of dendritic tracing or electrophysiological comparisons, approaches already limited by the small number of cells that can be evaluated. For population-wide studies, from neurotransmitter release to “-omics” approaches, each experiment would have to be normalized to reflect the current extent of mosaicism in each culture, dramatically confounding already variable analyses.
