Data are presented as means ± S.E.M. Preference and alcohol intake for the first three experimental days were compared using ANOVA with repeated measures. Comparisons between drug and saline treatments were carried out using analysis of variance (ANOVA) with drug treatment as the variable. The c-Fos data in Experiment 2 were analyzed using ANOVAs comparing the different treatment groups. For Experiment 3, a multi-factor ANOVA was carried out, with the first injection and the second injection as separate variables. When the interaction between the two injections was significant, only then was there considered to be an effect of the drug on ethanol-induced c-Fos. When there was no significant interaction between the two injections, data from the saline pre-treated mice or the D-Lys3-GHRP-6 pre-treated mice was pooled and compared using a Fisher’s PLSD post-hoc test. An effect was considered to be statistically significant when p < 0.05.
