Chunk #19 — Methods and Materials — Cell Counting

Source

The Creb1 coactivator Crtc1 is required for energy balance and fertility.

Embedded

yes

Text

P-STAT3 staining and nuclear and cytoplasmic TORC1 staining in hypothalamic sections were quantified by an independent observer in a blinded manner. Five to eight matched arcuate-containing sections from whole-brain series were counted from independent experiments.