We further performed unbiased hierarchical clustering of our data against previously published data on primary human brain cells isolated by immuno-panning, including neurons, fetal and mature astrocytes, and adult microglia 31. We find that pMGLs form a cluster with both fetal and mature primary microglia, while iPS-derived neural cells cluster with primary neurons and glia, close to fetal astrocytes (Figure 5b).
