Chunk #47 — Experimental Procedures — Microscopy

Source

Microfluidic local perfusion chambers for the visualization and manipulation of synapses.

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yes

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For FM5-95 dye loading, Kr laser excitation at 568 was used with long pass (BA585IF) and band pass (700BP) filters using a 60× water immersion lens (NA 1.2). For Fluo4-NW imaging the confocal aperture was opened to setting 4 and images were acquired continuously every 1.74 s.