The following oligonucleotides were SDS-PAGE purified and [P32] labeled with Klenow enzyme as described earlier [24]. Sequence of the plus strand of wild type (wt) and mutant (m)-κB fragments of the immunodeficiency virus enhancer [40] is shown; wt- and m-κB-sites are underlined and mutated nucleotides are indicated by bold letters: wt-κB 5′-GGTGATCAGGGACTTTCCGCTGGGGACTTTCCAGGAT-3′, m-κB 5′-GGTGATCATTC ACTTTCCGCTATTC ACTTTCCAGGAT-3′. Minus strands were extended 1 to 3 nucleotides from the 5′-end and recessed by 1-3 nucleotides from the 3′-end.
