highly distinct from the primary somatic cell lines, including the adult stem cell ADS line, and this relationship agrees with clustering of cell types based on transcriptional activity (Fig. 1e and Supplementary Fig. 1a, b). Analysis of DNA methylation patterns at enhancers, transcription-factor-binding sites and pluripotency-related genes confirmed the previously reported methylation patterns18 (Supplementary Figs 2–6). Taken together, these data indicate that, on the genome scale and at these crucial genomic regions, iPSC and ES cell DNA methylomes closely resemble one another.
