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Chunk #37 — Methods — Electrophysiology – patch clamp

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Sustained synchronized neuronal network activity in a human astrocyte co-culture system.
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The number of postsynaptic currents and the number and duration of quasi rhythmic events were analyzed using Clampfit (Molecular Devices). Cells were classified based on their activity pattern. Cells showing no sPSCs at all or up to five single sPCSs per minute were labeled with “no activity”, cells showing 5 or more single events or less than one burst per minute were labeled with “sparse activity” and cells bursting at a frequency higher than one burst per minute were labeled with “frequently bursting”. sPSC bursting frequency was defined as bursts per minute.